While this game uses assets, references and characters from the Teen Titans (2003) TV series, it does not explicitly say when it ties into the series continuity. However, there are many indications that the game is set after Teen Titans: Trouble in Tokyo (2006).
This game is a console game with 3D graphics created for the PlayStation 2, Nintendo GameCube, and Xbox. It was preceded by a Teen Titans game on Game Boy Advance in 2005 and later succeeded by another Game Boy Advance game, Teen Titans 2, released in late 2006.
This game features voice actor cast from the Teen Titans (2003) TV series reprising their roles. The only exception was Mad Mod who in this game features Greg Ellis performing the character instead of the TV series actor Malcolm McDowell.
This is the second game to feature Scott Menville performing as a version of Robin in a video game. Three years prior, Menville provided the voice of Robin/Tim Drake in the game Batman: Rise of Sin Tzu (2003) which is part of the DCAU continuity. While ambiguous in its first season, the continuity established in Teen Titans (2003), the show and continuity that this game is based on, is not part of the DCAU continuity. Furthermore, since its second season the TV series creative team have been dropping not-so-subtitle hints that the Robin featured in "Teen Titans" is the original Robin, Dick Grayson.
Slade in the game has his powers that were given to him by Trigon as seen in the fourth season of the Teen Titans (2003) TV series.